Fast skeletal myosin light chain 1 Antibody (MY-21) Summary
| Immunogen |
Chicken lens membranes.
|
| Localization |
Cytoplasmic
|
| Specificity |
Reacts in immunoblotting with the 20 kDa light chains of SDS, heat-denatured and reduced chicken gizzard, chicken intestine, and pig stomach myosin. Reacts with purified myosin light chains, but shows no reactivity in blotting with intact myosin molecules in their native state. Localizes an epitope on the stress fibers of well spread human and chicken fibroblasts by indirect immunofluorescence.
|
| Isotype |
IgM
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
MYL1
|
| Purity |
Unpurified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Dot: Use at an assay dependent dilution. The antibody reacts with purified myosin light chains (20,000 daltons) in both the native protein state or an SDS-heat denatured, reduced state. IF: Use at a dilution of 1/200. WB: Use at an assay dependent dilution. Detects a band of approximately 20 kDa.
|
Reactivity Notes
Cross-reacts with Human, Cow, Chicken, Pig and Rabbit. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
Ascites
|
| Preservative |
15mM Sodium Azide
|
| Purity |
Unpurified
|
Alternate Names for Fast skeletal myosin light chain 1 Antibody (MY-21)
- A1 catalytic
- A2 catalytic
- MLC1/MLC3
- MLC1F
- MLC1F/MLC3F
- MLC3F
- myosin light chain 1/3, skeletal muscle isoform
- Myosin light chain A1/A2
- Myosin light chain alkali 1/2
- myosin, light chain 1, alkali; skeletal, fast
- myosin, light polypeptide 1, alkali; skeletal, fast
Background
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.