FLIP Antibody Summary
| Immunogen |
Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP. The immunogen is located within amino acids 180 – 230 of FLIP.
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| Specificity |
Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
CFLAR
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.
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| Theoretical MW |
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for FLIP Antibody
- CASHFADD-like antiapoptotic molecule 1
- CASP8 and FADD-like apoptosis regulator
- CASP8AP1
- Caspase homolog
- Caspase-eight-related protein
- Caspase-like apoptosis regulatory protein
- caspase-related inducer of apoptosis
- CASPER
- CasperFLAME1
- Cellular FLICE-like inhibitory protein
- CFLAR
- c-FLIPR
- c-FLIPS
- CLARPMACH-related inducer of toxicity
- FADD-like apoptotic molecule
- FLAME
- FLAME-1
- FLIP
- I-FLICE
- I-FLICEMRITc-FLIPFLIPc-FLIPL
- Inhibitor of FLICE
- usurpin beta
- Usurpin
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively. The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.