DFF40/CAD Antibody Summary
| Immunogen |
Antibody was raised against a 16 amino acid peptide near the center of human DFF40. The immunogen is located within amino acids 190 – 240 of DFF40.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
DFFB
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
DFF40 antibody can be used for detection of DFF40 /CAD by Western blot at 1:500 dilution. A 40 kDa band can be detected. It is human, mouse, and rat reactive,
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| Theoretical MW |
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for DFF40/CAD Antibody
- CAD
- CADCaspase-activated nuclease
- Caspase-activated deoxyribonuclease
- Caspase-activated DNase
- CPAN
- CPANDNA fragmentation factor 40 kDa subunit
- DFF2
- DFF40
- DFF-40DFF40DFF2DNA fragmentation factor subunit beta
- DFFB
- DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
- EC 3.-
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.