MHC Class I Antibody (ERMP42) Summary
| Immunogen |
murine macrophage precursor cells
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| Specificity |
NB120-15680 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class I molecule. In the mouse, the MHC class I molecule is expressed on the cell surface of all nucleated cells but expression levels may differ according to cell type. Clone ER-MP42 specifically recognizes mouse strains with the haplotypes H-2Kv, H-2Dd and H-2k,q,s. Mouse strains with the haplotypes H-2p,r,w7,w22 show weak reactivity with this antibody.
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| Isotype |
IgG2a
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| Clonality |
Monoclonal
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| Host |
Rat
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| Gene |
HLA-E
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
IHC-P: Does not react on routinely fixed paraffin sections (4% formalin). Use at a fixation of 1% formalin or glutaraldehyde). Optimal dilutions/concentrations should be determined by the end user. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for MHC Class I Antibody (ERMP42)
- DKFZp686P19218
- EA1.2
- EA2.1
- HLA class I histocompatibility antigen, alpha chain E
- HLA class I histocompatibility antigen, E alpha chain
- HLA-6.2
- HLAE
- lymphocyte antigen
- major histocompatibility complex, class I, E
- MHC class I antigen E
- MHC HLA-E alpha-1
- MHC HLA-E alpha-2.1
- MHC
- QA1