Granzyme B Antibody (GB11) Summary
| Immunogen |
Purified human Granzyme B (GZMB)
|
| Specificity |
NB100-64774 recognizes the serine protease Granzyme B (GZMB)
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
GZMB
|
| Purity |
Protein A purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
For Flow Cytometry: Membrane permeabilisation is required for this application. Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. In ELISA, this product has been used as a capture antibody with NB100-62617 used as the detection antibody.
|
Reactivity Notes
Cross reacts with Chimpanzee, Monkey and Rhesus Monkey.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
TBS
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein A purified
|
Alternate Names for Granzyme B Antibody (GB11)
- CSPB
- CTLA-1
- CTSGL1
- Fragmentin-2
- Granzyme B
- Granzyme-2
- GRB
- GrzB
- GZMB
- HLP
- SECT
Background
Granzyme B is a cysteine protease found in the cytoplasmic lysosome-like granules of cytolytic T lymphocytes (CTL) and natural killer (NK) cells and is is required for the induction of target cell lysis. This occurs as part of cell mediated immune responses, and can activate apoptosis in target cells by both caspase dependent and caspase independent mechanisms.