Actin Alpha 1 Skeletal Muscle Antibody (5C5) Summary
| Immunogen |
The purified rabbit striated muscle actin.
|
| Specificity |
Monoclonal anti-ALPHA-sarcomeric actin has been used as a marker for rhabdomyosarcoma. This reacts with a-skeletal and a-cardiac muscle actins. It does not react with smooth muscle tissue.
|
| Isotype |
IgM
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
ACTA1
|
| Purity |
Unpurified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
|
| Application Notes |
This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation inbuffered formalin can destroy the epitope. It is recommended that this product be used on frozen tissue sectionsor specimens. The optimal conditions should be determined by the individual laboratory. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26567220)
|
|
| Publications |
|
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 26567220). Cross-reacts with sheep, bovine, guinea pig, rat, frog, snake and carp. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
Ascites
|
| Preservative |
Sodium Azide
|
| Purity |
Unpurified
|
Alternate Names for Actin Alpha 1 Skeletal Muscle Antibody (5C5)
- ACTA
- actin, alpha 1, skeletal muscle
- alpha skeletal muscle actin
- alpha skeletal muscle
- alpha-actin-1
- ASMA
- CFTD
- CFTDM
- MPFD
- NEM1
- NEM2
- NEM3
Background
Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4).