Monomethyl Arginine Antibody (5D1)

Product: Protoporphyrin IX

Monomethyl Arginine Antibody (5D1) Summary

Immunogen
Modified monomethyl arginine
Specificity
This reacts with free and bound NG-monomethyl arginine. There is no cross reaction with either NG-NG-dimethyl arginine or arginine.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:100 – 1:10 000
  • Immunohistochemistry-Paraffin 1:100-1:10000
Publications
Read Publication using NB500-122.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris, 0.1M Glycine and 2% Sucrose
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml distilled water.

Alternate Names for Monomethyl Arginine Antibody (5D1)

  • Arginine (mono methyl)
  • Di methyl Arginine
  • Dimethyl Arginine
  • Methyl Arginine
  • Mono methyl Arginine
  • Monomethyl Arginine

Background

This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognizes monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

PMID: 26617570