PMCA2 Antibody Summary
| Immunogen |
Synthetic Peptide: T(5) N S D F Y S K N Q R N E S S(19)
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| Specificity |
PMCA2 antibody detects calcium pump of the plasma membrane 2 (PMCA2 ATPase) from human and rat and porcine tissues and cells.
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| Isotype |
IgG
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
ATP2B2
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Detects an approx. 127 kDa and 133 kDa proteins representing PMCA 2a and 2b ATPase from rat brain microsomal fractions.
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| Positive Control |
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Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS, 1 mg/ml BSA
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| Preservative |
0.05% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for PMCA2 Antibody
- plasma membrane calcium-transporting ATPase 2
- ATPase, Ca++ transporting, plasma membrane 2
- PMCA2
- PMCA2a
- PMCA2i
Background
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin.