Human Adrenal Whole Tissue Lysate (Adult Whole Normal)

Product: Promazine (hydrochloride)

Human Adrenal Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein – Human Adult Normal Tissue: Adrenal

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Positive Control
Connexin 43/GJA1 Antibody (NB100-81868)
CYP11B1 Antibody (NBP1-68883)
CYP21A2 Antibody (NBP2-13893)
Connexin 43/GJA1 Antibody (NBP1-67530)

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCL, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Concentration
5.0 mg/ml

Lysate Details for Adrenal

Type
Tissue
Life Stage
Adult
Tissue
Endocrine
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

PMID: 20855444