AIF Antibody Summary
| Immunogen |
Rabbit anti-AIF (IN) polyclonal antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF (2). This sequence is identical to those of mouse and rat AIF (2).
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
AIFM1
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| Purity |
Ion exchange chromatography
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This polyclonal antibody can be used for detection of AIF by Western blot. K562 cell lysate can be used as positive control and a 67 kDa band should be detected. It can also be used to detect AIF by Immunohistochemistry-Paraffin.
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| Positive Control |
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Reactivity Notes
This antibody is reactive against Human, Mouse, Rat
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
2.0 mg/ml
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| Purity |
Ion exchange chromatography
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Alternate Names for AIF Antibody
- AIF
- AIFapoptosis-inducing factor 1, mitochondrial
- AIFM1
- apoptosis-inducing factor, mitochondrion-associated, 1
- COXPD6
- MGC111425
- PDCD8
- PDCD8striatal apoptosis-inducing factor
- programmed cell death 8 (apoptosis-inducing factor)
- Programmed cell death protein 8
Background
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and widely expressed (2).