Von Willebrand Factor Antibody (21-43)

Product: Azoramide

Von Willebrand Factor Antibody (21-43) Summary

Immunogen
Purified Human Factor VIII complex
Marker
Endothelial Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VWF
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Radioimmunoassay 1:100-1:2000
Application Notes
This antibody reacts strongly with human von Willebrand Factor or Factor VIII Related Antigen in both plasma, using RIA or ELISA, and in tissues by immunofluorescence or immunoperoxidase staining. The antibody is suitable for use with frozen and formalin fixed tissue sections. Detecta a band at ~310 by Western Blot.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 0.2% BSA and 50% (v/v) Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.4 mg/ml
Purity
Protein A purified

Alternate Names for Von Willebrand Factor Antibody (21-43)

  • coagulation factor VIII VWF
  • F8
  • F8VWF
  • von Willebrand factor
  • VWD
  • vWF

Background

von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis / neovascularization.

PMID: 21029459