Recombinant Human Arginase 1/ARG1/liver Arginase Protein Summary
| Description |
A recombinant protein with a His tag and corresponding to amino acids 1 – 322 of ARG1.
Source: E. coli Amino Acid Sequence: MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
ARG1
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
35.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Notes
The purity of this protein is > 85% by SDS-PAGE. Molecular weight is 35.8kDa (330aa), confirmed by MALDI-TOF
Alternate Names for Recombinant Human Arginase 1/ARG1/liver Arginase Protein
- ARG1
- Arginase 1
- arginase, liver
- arginase-1
- EC 3.5.3.1
- Liver-type arginase
- Type I arginase
Background
Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate. Recombinant human ARG1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.