Recombinant Human Adenylate Kinase 1 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 194 of Human Adenylate Kinase 1
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity: > 600 units/mg. One unit will convert 2.0 umolesof ADP to ATP + AMP per minute at pH 7.5 at 37C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
AK1
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 23.7 kDa (214aa) confirmed by MALDI-TOF
Assay |
| Theoretical MW |
23.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20 mM Tris-HCl Buffer (pH 7.5) containing 10% glycerol
|
| Concentration |
1 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Human Adenylate Kinase 1 Protein
- adenylate kinase 1
- adenylate kinase isoenzyme 1
- AK 1
- ATP-AMP transphosphorylase 1
- EC 2.7.4
- EC 2.7.4.3
- myokinase
Background
AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.