Product: N-acetyl Dapsone (D5)
Recombinant Human Meprin alpha Subunit/MEP1A Protein Summary
| Description |
22-601aa His-tag Baculovirus
Amino Sequence: ADPVPIKYLP EENVHDADFG EQKDISEINL AAGLDLFQGD ILLQKSRNGL RDPNTRWTFP IPYILADNLG LNAKGAILYA FEMFRLKSCV DFKPYEGESS YIIFQQFDGCWSEVGDQHVG QNISIGQGCA YKAIIEHEIL HALGFYHEQS RTDRDDYVNI WWDQILSGYQ HNFDTYDDSL ITDLNTPYDY ESLMHYQPFS FNKNASVPTI TAKIPEFNSIIGQRLDFSAI DLERLNRMYN CTTTHTLLDH CTFEKANICG MIQGTRDDTD WAHQDSAQAG EVDHTLLGQC TGAGYFMQFS TSSGSAEEAA LLESRILYPK RKQQCLQFFYKMTGSPSDRL VVWVRRDDST GNVRKLVKVQ TFQGDDDHNW KIAHVVLKEE QKFRYLFQGT KGDPQNSTGG IYLDDITLTE TPCPTGVWTV RNFSQVLENT SKGDKLQSPRFYNSEGYGFG VTLYPNSRES SGYLRLAFHV CSGENDAILE WPVENRQVII TILDQEPDVR NRMSSSMVFT TSKSHTSPAI NDTVIWDRPS RVGTYHTDCN CFRSIDLGWSGFISHQMLKR RSFLKNDDLI IFVDFEDITH LSQHHHHHH Molecular Weight: (589aa)70-100kDa (SDS-PAGE under reducing conditions) |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
MEP1A
|
| Purity |
>85%, by SDS-PAGE
|
| Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method)
|
Applications/Dilutions
| Theoretical MW |
67.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
|
| Concentration |
0.25 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Alternate Names for Recombinant Human Meprin alpha Subunit/MEP1A Protein
- bA268F1.1 (meprin A alpha (PABA peptide hydrolase))
- EC 3.4.24
- EC 3.4.24.18
- endopeptidase-2
- MEP1A
- meprin A subunit alpha
- meprin A, alpha (PABA peptide hydrolase)
- Meprin alpha Subunit
- N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha
- PABA peptide hydrolase
- PPH alpha
- PPHA
Background
MEP1A, also known as meprin A subunit alpha, is a single-pass type I membrane protein which belongs to the peptidase M12A family. It abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. It is capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Recombinant human MEP1A, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.