Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein Summary
| Description |
A recombinant protein with a His tag and corresponding to amino acids 1 – 323 of AKR1C1.
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
AKR1C1
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
1.Prepare 180 ml assay buffer into a sutbale container: the concentrations are 50 mM Tris-HCl (pH 8.8), 250 mM NaCl, 0.4 mM NADP, 1 mM 1-acenaphthenol.
2. Equilibroate to 25C and monitor at A340 until the valiue is consistant using a spectrophotometer. 3. Add 20 ul of recombinant human Aldo-keto Reductase 1C1/AKR1C1 Protein 250 ug/ml in 50 mM Tris-HCl (pH 8.8), 250 mM NaCl, and 0.1% BSA. 4. Record in A340 nm for 5 minutes. |
| Theoretical MW |
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 38.9 kDa (343aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human Aldo-keto Reductase 1C1/AKR1C1 Protein
- 20 alpha-hydroxysteroid dehydrogenase
- 20-ALPHA-HSD
- 20-alpha-hydroxysteroid dehydrogenase
- 2-ALPHA-HSD
- AKR1C1
- Aldo-keto Reductase 1C1
- aldo-keto reductase family 1 member C1
- aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha(3-alpha)-hydroxysteroid dehydrogenase)
- AldoketoReductase 1C1
- C9
- Chlordecone reductase homolog HAKRC
- DD1/DD2
- DD1MGC8954
- DDH1
- DDHH-37
- dihydrodiol dehydrogenase 1
- Dihydrodiol dehydrogenase 1/2
- dihydrodiol dehydrogenase isoform DD1
- EC 1.1.1
- EC 1.1.1.-
- EC 1.1.1.112
- EC 1.1.1.149,2-ALPHA-HSD
- EC 1.3.1.20
- HAKRC
- HAKRCDDH1aldo-keto reductase C
- HBAB
- hepatic dihydrodiol dehydrogenase
- High-affinity hepatic bile acid-binding protein
- Indanol dehydrogenase
- MBAB
- Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
- type II 3-alpha-hydroxysteroid dehydrogenase
Background
AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E.coli and purified by using conventional chromatography techniques.