Recombinant Human Lymphotoxin-alpha/TNF-beta Protein Summary
| Description |
A recombinant protein with a His tag and corresponding to amino acids 35 – 205 of LTA.
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL |
| Preparation Method |
E.coli
|
| Details of Functionality |
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 1 ng/ml.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
LTA
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
Assay
L929 cytotoxicity assay using actinomycin D Cell Number: 2 x 10,000 cells/well Incubation: 24hr after sample treatment Assay Media: 5% FBS media Cytokine Concentration: 12 pg/ml – 25 ng/ml Detection method: MTT assay |
| Theoretical MW |
20.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Human protein.
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 20.9 kDa (192aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human Lymphotoxin-alpha/TNF-beta Protein
- LT
- LTA
- LT-alpha
- lymphotoxin alpha (TNF superfamily, member 1)
- Lymphotoxin alpha
- Lymphotoxinalpha
- Lymphotoxin-alpha
- tnfb
- TNF-beta
- TNFBlymphotoxin-alpha
- TNFSF1
- TNFSF1B
- TNFSF1TNF-beta
- tumor necrosis factor beta
- Tumor necrosis factor ligand superfamily member 1
Background
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant TNF beta protein was expressed in E.coli and purified by using conventional chromatography techniques.