Recombinant Human Guanine deaminase Protein Summary
| Description |
An un-tagged recombinant protein corresponding to amino acids 1 to 454 of Human Guanine deaminase
Source: E.coli Amino Acid Sequence: MCAAQMPPLA HIFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEEASQ QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHASQYSF AGSSIDLPLL EWLTKYTFPA EHRFQNIDFA EEVYTRVVRR TLKNGTTTAC YFATIHTDSS LLLADITDKF GQRAFVGKVC MDLNDTFPEY KETTEESIKE TERFVSEMLQ KNYSRVKPIV TPRFSLSCSE TLMGELGNIA KTRDLHIQSH ISENRDEVEA VKNLYPSYKN YTSVYDKNNL LTNKTVMAHG CYLSAEELNV FHERGASIAH CPNSNLSLSS GFLNVLEVLK HEVKIGLGTD VAGGYSYSML DAIRRAVMVS NILLINKVNE KSLTLKEVFR LATLGGSQAL GLDGEIGNFE VGKEFDAILI NPKASDSPID LFYGDFFGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF SSSV |
| Details of Functionality |
Specific activity is > 2,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
GDA
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 85% as determined by SDS-PAGE. Molecular weight is 51kDa.
|
| Theoretical MW |
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Human
Packaging, Storage & Formulations
| Storage |
Store at -80C in the dark. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
|
| Concentration |
1 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human Guanine deaminase Protein
- CYPIN
- EC 3.5.4.3
- GAH
- GUANASE
- Guanine aminase
- Guanine aminohydrolase
- guanine deaminase
- KIAA1258
- MGC9982
- NEDASIN
- p51-nedasin
Background
GDA, also known as guanine deaminase isoform b, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA, was expressed in E.coli and purified by conventional chromatography techniques. Produced in E. coli