Aurora B Antibody (2B1) Summary
| Immunogen |
Recombinant human Aurora kinase B (1-344aa) purified from E. coli
|
| Marker |
Mitosis Marker
|
| Specificity |
Anti-human Aurora kinase B mAb, clone 2B1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Aurora kinase B protein.
|
| Isotype |
IgG2b Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
AURKB
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
The antibody has been tested by ELISA and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Immunofluorescence is 1:1000 ~ 2000. Recommended starting dilution is 1:1000
|
Reactivity Notes
Human
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4) and 10% Glycerol
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein G purified
|
Alternate Names for Aurora B Antibody (2B1)
- Aik2
- AIK2EC 2.7.11.1
- AIM-1
- AIM1Aik2
- AIM-1STK-1
- ARK2
- ARK2ARK-2
- AurB
- AURKB
- aurkb-sv2
- Aurora- and IPL1-like midbody-associated protein 1
- Aurora B
- aurora kinase BSerine/threonine-protein kinase aurora-B
- aurora kinase B-Sv1
- aurora kinase B-Sv2
- Aurora/IPL1-related kinase 2
- aurora-1
- aurora-B
- Aurora-related kinase 2
- EC 2.7.11
- IPL1
- serine/threonine kinase 12
- serine/threonine-protein kinase 12
- STK12
- STK12aurkb-sv1
- STK5
Background
The aurora family (A, B and C) are serine threonine kinases and key regulators of chromosome segregation during mitosis. Aurora kinase B is a chromosomal passenger protein that regulates chromosome segregation and cytokinesis. Aurora kinase B is associated with the level of genetic instability within tumours and patient survival. It is strongly expressed in exponentially proliferating bronchial epithelial cells in culture and that this expression is markedly reduced in confluent cells. It is also shown that almost all tumours show higher levels of Aurora kinase B expression than their matched normal lung tissues, which could therefore simply be a consequence of a higher proliferative index, or be typical of the progenitor cell and atypical of the bulk of normal lung cells