Product: Endoxifen (E-isomer hydrochloride)
Recombinant Human Cyclophilin B Protein Summary
| Description |
An un-tagged recombinant protein corresponding to amino acids 26 to 216 of Human Cyclophilin B
Source: E.coli Amino Acid Sequence: MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
PPIB
|
| Purity |
>95% pure by SDS-PAGE
|
| Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method)
|
Applications/Dilutions
| Application Notes |
Assay
1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin. 2. Add 10ul of recombinant Cyclophilin B protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5. Record the increase in A405 nm for 30 minutes at 25C. |
|
| Theoretical MW |
21.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris 8.0,.20mM NaCl, 0.5mM DTT, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 21.2kDa (192aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human Cyclophilin B Protein
- Cyclophilin B
- cyclophilin-like protein
- CYPB
- CYPBEC 5.2.1.8
- CYP-S1
- MGC2224
- OI9MGC14109
- peptidyl-prolyl cis-trans isomerase B
- peptidylprolyl isomerase B (cyclophilin B)
- PPIase B
- PPIB
- Rotamase B
- S-cyclophilin
- SCYLP
Background
Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E.coli and purified by using conventional chromatography techniques