Recombinant Human Gremlin 1 Protein Summary
| Description |
A denatured recombinant protein with a His tag corresponding to amino acids 25 – 184 of GREM1.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSKKKGSQG AIPPPDKAQH NDSEQTQSPQ QPGSRNRGRG QGRGTAMPGE EVLESSQEAL HVTERKYLKR DWCKTQPLKQ TIHEEGCNSR TIINRFCYGQ CNSFYIPRHI RKEEGSFQSC SFCKPKKFTT MMVTLNCPEL QPPTKKKRVT RVKQCRCISI DLD |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
GREM1
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human Gremlin 1 Protein
- Cell proliferation-inducing gene 2 protein
- CKTSF1B1gremlin 1, cysteine knot superfamily, homolog
- Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis)
- DAN domain family member 2
- DAND2
- DAND2GREMLIN
- Down-regulated in Mos-transformed cells protein
- DRM
- DRMMGC126660
- GREM1
- gremlin 1
- gremlin 1-like protein
- Gremlin
- gremlin-1
- IHG-2
- Increased in high glucose protein 2
- increased in high glucose-2
- proliferation-inducing gene 2
Background
GREM1 is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this protein belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this protein may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Recombinant human GREM1 protein, fused to His-tag at N-terminus, was expressed in E.coli.