Recombinant Human EMP/MAEA Protein Summary
| Description |
A denatured recombinant protein with a His tag corresponding to amino acids 1 – 396 of MAEA.
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAVQESA AQLSMTLKVQ EYPTLKVPYE TLNKRFRAAQ KNIDRETSHV TMVVAELEKT LSGCPAVDSV VSLLDGVVEK LSVLKRKAVE SIQAEDESAK LCKRRIEHLK EHSSDQPAAA SVWKRKRMDR MMVEHLLRCG YYNTAVKLAR QSGIEDLVNI EMFLTAKEVE ESLERRETAT CLAWCHDNKS RLRKMKSCLE FSLRIQEFIE LIRQNKRLDA VRHARKHFSQ AEGSQLDEVR QAMGMLAFPP DTHISPYKDL LDPARWRMLI QQFRYDNYRL HQLGNNSVFT LTLQAGLSAI KTPQCYKEDG SSKSPDCPVC SRSLNKLAQP LPMAHCANSR LVCKISGDVM NENNPPMMLP NGYVYGYNSL LSIRQDDKVV CPRTKEVFHF SQAEKVYIM |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
MAEA
|
| Purity |
>85%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
47.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 1M urea, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
0.25 mg/ml
|
| Purity |
>85%, by SDS-PAGE
|
Alternate Names for Recombinant Human EMP/MAEA Protein
- EMLP
- EMP
- EMPCell proliferation-inducing gene 5 protein
- Erythroblast macrophage protein
- HLC-10
- Human lung cancer oncogene 10 protein
- lung cancer-related protein 10
- macrophage erythroblast attacher
- MAEA
- PIG5
- proliferation-inducing gene 5
Background
Macrophage erythroblast attacher isoform 1, also known as MAEA, is a 396 amino acid ubiquitously expressed adhesion protein. Expressed as 5 alternatively spliced isoforms, MAEA contains one CTLH domain and one LisH domain. MAEA can form a complex with F-actin, which is involved regulating actin distribution in erythroblasts and macrophages. Considered to assist with cell division and nuclear architecture, MAEA is localized with condensed chromatin at prophase, nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis. Recombinant human MAEA protein, fused to His-tag at N-terminus, was expressed in E.coli.