NPM1 Antibody (5E3) Summary
| Immunogen |
Recombinant human NPM (81-294aa) purified from E. coli
|
| Specificity |
Anti-human NPM mAb, clone 5E3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NPM protein.
|
| Isotype |
IgG2b
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
NPM1
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
The antibody has been tested by ELISA and western blotting analysis to assure specificity and reactivity.
|
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4) and 10% Glycerol
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1.0 mg/ml
|
| Purity |
Protein G purified
|
Alternate Names for NPM1 Antibody (5E3)
- B23
- MGC104254
- NPM1
- NPMnucleophosmin/nucleoplasmin family, member 1
- Nucleolar phosphoprotein B23
- Nucleolar protein NO38
- nucleophosmin (nucleolar phosphoprotein B23, numatrin)
- Nucleophosmin
- Numatrin
Background
Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.