MMR/CD206/Mannose Receptor Antibody (15-2) Summary
| Immunogen |
Purified human mannose receptor from human placental tissue
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
MRC1
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
For IHC-F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum. For FC: Antibody 15-2 stains the extracellular domain of MR. WB: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa. Functional Studies: Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor.
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Reactivity Notes
Human. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS containing 0.1% BSA
|
| Preservative |
0.02% Sodium Azide
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| Concentration |
0.1 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for MMR/CD206/Mannose Receptor Antibody (15-2)
- CD206
- CLEC13D
- CLEC13Dmacrophage mannose receptor 1
- C-type lectin domain family 13 member D
- mannose receptor, C type 1
- MMR
- MMRCD206 antigen
- MRC1
Background
The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MRs are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.