GABA-A R delta Antibody (S151-3) Summary
| Immunogen |
Apparent mol. wt of 97kD, non-glycosylated version at 68kD. Other minor bands associated with hNIS at160 kDa, and degradation products at approx. 30 kDa, and approx. 15kDa.Synthetic peptide amino acids 15-34 of rat GABA-A-R-Delta
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| Localization |
Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane
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| Specificity |
Detects approx 55kDa.
|
| Isotype |
IgG2a
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| Clonality |
Monoclonal
|
| Host |
Mouse
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| Gene |
GABRD
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| Purity |
Protein G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
2 ug/ml of GABA-A Receptor Delta Antibody was sufficient for detection of Delta1 GABA-A receptor in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
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Reactivity Notes
Based on homology, Its predicted to detect Human and Mouse.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.4) and 50% Glycerol
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Protein G purified
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Alternate Names for GABA-A R delta Antibody (S151-3)
- EIG10
- EJM7
- GABA A R delta
- GABA(A) receptor subunit delta
- GABA-A receptor, delta polypeptide
- GABAAR delta
- GABAARd
- GABRD
- gamma-aminobutyric acid (GABA) A receptor, delta
- gamma-aminobutyric acid receptor subunit delta
- GEFSP5
- MGC45284
Background
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003). More recently there have been a number of studies demonstrating that the alpha-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).