DUX4 Antibody (P2B1)

Product: Fumarate hydratase-IN-3 (sodium salt)

DUX4 Antibody (P2B1) Summary

Immunogen
This antibody recognizes human DUX4 C-terminal 76 amino acids of DUX4 with glutathione-s-transferase (gst) tag.It does not cross react with DUX4c.
Localization
Nucleus
Specificity
Detects approx 45kDa. No cross-reactivity with DUX4c.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DUX4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
1 ug/ml of DUX4 Antibody was sufficient for detection of DUX4 in 20 ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as the secondary.

Reactivity Notes

Based on homology, Its predicted to detect Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for DUX4 Antibody (P2B1)

  • double homeobox 4
  • double homeobox protein 10
  • double homeobox protein 4
  • double homeobox protein 4/10
  • double homeobox protein DUX10
  • DUX10

Background

1ug/mL was sufficient for detection of DUX4 in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG: HRP as teh secondary.

PMID: 17643112