SHANK2 Antibody (S23b-49)

Product: Alprenolol

SHANK2 Antibody (S23b-49) Summary

Immunogen
Fusion protein amino acids 84-309 of rat Shank2
Localization
Cytoplasm, Cell Junction, Synapse, Postsynaptic Cell Membrane, Postsynaptic Density, Cell Projection, Growth Cone
Specificity
Detects approx 160kDa. Recognizes Shank1, 2 and 3.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SHANK2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of SHANK (pan) Antibody was sufficient for detection of Shank1-4 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, Its predicted to detect Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SHANK2 Antibody (S23b-49)

  • AUTS17
  • cortactin binding protein 1
  • cortactin SH3 domain-binding protein
  • Cortactin-binding protein 1
  • CORTBP1
  • CORTBP1CTTNBP1
  • CTTNBP1
  • GKAP/SAPAP interacting protein
  • KIAA1022
  • proline-rich synapse associated protein 1
  • Proline-rich synapse-associated protein 1
  • ProSAP1
  • SH3 and multiple ankyrin repeat domains 2
  • SH3 and multiple ankyrin repeat domains protein 2
  • SHANK
  • SHANK2
  • SPANK-3

Background

1 ug/mL was sufficient for detection of Shank1-4 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

PMID: 27570067