Non-species specific 8-OHdG ELISA Kit Summary
| Standard Curve Range |
0.94 – 60 ng/mL
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| Sensitivity |
0.59 ng/mL
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| Assay Type |
Competitive ELISA
|
| Intra-Assay |
Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.Inter-Assay Precision: Three samples of known concentration were assayed thirty ti
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| Kit Type |
ELISA Kit
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Applications/Dilutions
| Application Notes |
ELISA Kit for 8-OHdG detection in samples.
No of Samples: 39 samples in duplicate Incubation Time: 1 hour |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Alternate Names for Non-species specific 8-OHdG ELISA Kit
- 8 OHG
- 8-hydroxy-2
- 8-hydroxydeoxyguanosine
Background
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).