DDX19B Antibody

Product: 2-Methoxyestradiol

DDX19B Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX19B. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus; nuclear pore complex; Nucleus membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX19B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1.02 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX19B Antibody

  • ATP-dependent RNA helicase DDX19
  • DBP5ATP-dependent RNA helicase DDX19B
  • DDX19DEAD-box protein 5
  • DEAD (Asp-Glu-Ala-As) box polypeptide 19
  • DEAD (Asp-Glu-Ala-As) box polypeptide 19B
  • DEAD box protein 19B
  • DEAD box RNA helicase DEAD5
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog)
  • DEAD-box RNA helicase DEAD5
  • EC 3.6.1
  • EC 3.6.4.13
  • RNAh
  • yeast Dbp5 homolog

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

PMID: 18695638