dUTPase Antibody Summary
| Immunogen |
Recombinant protein encompassing a sequence within the center region of human DUT. The exact sequence is proprietary.
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| Isotype |
IgG
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
DUT
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Expected cross reactivity based on sequence homology: Mouse (85%), Rat (85%).
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
| Buffer |
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
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| Preservative |
0.01% Thimerosal
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| Concentration |
0.49 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for dUTPase Antibody
- deoxyuridine triphosphatase
- dUTP pyrophosphatasedUTP nucleotidohydrolase
- dUTPasedeoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial
- EC 3.6.1.23
- FLJ20622
Background
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq]