Recombinant IGF-II Protein Summary
| Description |
A recombinant protein corresponding to human IGF-II.
Source: E. coli Amino Acid Sequence: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE |
| Details of Functionality |
The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 105 IU/mg.
|
| Source |
E. coli
|
| Protein/Peptide Type |
Biologically Active Protein
|
| Gene |
IGF2
|
| Purity |
> 98 % pure by SDS-PAGE and HPLC
|
| Endotoxin Note |
Less than 1 EU/ug of rHuIGF-2 as determined by LAL method.
|
Applications/Dilutions
| Theoretical MW |
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20 °C.
|
| Buffer |
20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20.
|
| Preservative |
No Preservative
|
| Concentration |
LYOPH
|
| Purity |
> 98 % pure by SDS-PAGE and HPLC
|
| Reconstitution Instructions |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
|
Alternate Names for Recombinant IGF-II Protein
- C11orf43
- chromosome 11 open reading frame 43
- FLJ22066
- FLJ44734
- IGF2
- IGF-2
- IGFII
- IGF-II
- insulin-like growth factor 2 (somatomedin A)
- insulin-like growth factor II
- insulin-like growth factor type 2
- MSA
- PP9974
- somatomedin-A