c-Abl [p Tyr412] Antibody

Product: MK 0893

c-Abl [p Tyr412] Antibody Summary

Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Abl 1b that contains tyrosine 412. Note: there are two widely expressed forms of c-Abl produced by alternative splicing, designated 1b (the more common form) and 1a. The corresponding phosphorylation site from 1a is tyrosine 393.
Modification
p Tyr412
Localization
Cytoplasmic and Nuclear
Specificity
c-Abl [Tyr412]
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
ABL1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
WB: Use at a concentration of 0.1 – 1.0 ug/ml. Detects a band of approximately 140 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Positive Control
c-Abl Lysate (NBL1-07208)
Publications
Read Publication using
NSB252 in the following applications:

  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 23818519).

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Dulbeccos PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Abl [p Tyr412] Antibody

  • Abelson murine leukemia viral oncogene homolog 1
  • ABL
  • ABL1
  • bcr/abl
  • BCR-ABL1
  • c-abl oncogene 1, non-receptor tyrosine kinase
  • c-abl oncogene 1, receptor tyrosine kinase
  • cAbl
  • c-Abl
  • EC 2.7.10
  • EC 2.7.10.2
  • JTK7
  • JTK7bcr/abl
  • p150bcr/c-abl oncogene protein
  • Proto-oncogene c-Abl
  • proto-oncogene tyrosine-protein kinase ABL1
  • tyrosine-protein kinase ABL1
  • v-abl Abelson murine leukemia viral oncogene homolog 1
  • v-abl

Background

c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.

PMID: 9187336