FADS1 Antibody

Product: R406 (free base)

FADS1 Antibody Summary

Immunogen
Peptide with sequence C-QPSFEPTKNKELTDE corresponding to internal region according to NP_037534.3.
Epitope
QPSFEPTKNKELTDE
Clonality
Polyclonal
Host
Goat
Gene
FADS1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 – 1 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 50 kDa band observed in human liver and lung lysates (calculated MW of 51.9 kDa band according to NP_037534.2).
Positive Control
Lung Lysate (NB820-59235)
FADS1 Lysate (NBL1-10425)
Publications
Read Publication using NB100-57083.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FADS1 Antibody

  • D5DFLJ90273
  • Delta(5) desaturase
  • Delta(5) fatty acid desaturase
  • Delta-5 desaturase
  • delta-5 fatty acid desaturase
  • EC 1.14.19
  • EC 1.14.19.-
  • FADS6
  • FADSD5LLCDL1
  • fatty acid desaturase 1
  • FLJ38956
  • linoleoyl-CoA desaturase (delta-6-desaturase)-like 1
  • TU12

Background

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

PMID: 26527057

FADS1 Antibody

Product: PD-1-IN-2

FADS1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human FADS1. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FADS1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 – 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (88%), Porcine (88%), Rat (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.91 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FADS1 Antibody

  • D5DFLJ90273
  • Delta(5) desaturase
  • Delta(5) fatty acid desaturase
  • Delta-5 desaturase
  • delta-5 fatty acid desaturase
  • EC 1.14.19
  • EC 1.14.19.-
  • FADS6
  • FADSD5LLCDL1
  • fatty acid desaturase 1
  • FLJ38956
  • linoleoyl-CoA desaturase (delta-6-desaturase)-like 1
  • TU12

Background

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq]

PMID: 18029031