NLRP3/NALP3 Antibody Summary
| Immunogen |
Peptide with sequence C-DLYEKAKRDEPK corresponding to internal region according to NP_004886.3, NP_899632.1, NP_001230062.1, NP_001120933.1, NP_001120934.1.
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| Specificity |
This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).
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| Clonality |
Polyclonal
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| Host |
Goat
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| Gene |
NLRP3
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| Purity |
Immunogen affinity purified
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| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Preliminary experiments gave an approx. 70 kDa band in human lymph node and tonsil lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118 kDa band according to NP_004886.3 and 106 kDa band according to NP_899632.1. The 70 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts.
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| Positive Control |
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| Publications |
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Reactivity Notes
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Notes
Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.
Alternate Names for NLRP3/NALP3 Antibody
- AGTAVPRL
- AII
- AII/AVP
- Angiotensin/vasopressin receptor AII/AVP-like
- AVP
- C1orf7
- Caterpiller protein 1.1
- CIAS1
- CLR1.1
- Cold autoinflammatory syndrome 1 protein
- Cold autoinflammatory syndrome 1
- Cryopyrin
- Familial cold autoinflammatory syndrome
- FCAS
- FCU
- Leucine-rich repeat-, and PYD-containing protein 3
- Muckle-Wells syndrome
- MWS
- NACHT
- NACHT, LRR and PYD containing protein 3
- NACHT, LRR and PYD domains-containing protein 3
- NALP3
- NLR family, pyrin domain containing 3
- NLRP3
- Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3
- PYPAF1
- PYRIN-containing APAF1-like protein 1
Background
This antibody is expected to recognise both isoform a (NP_004886.3 and NP_001073289.1) and isoform b (NP_899632.1). Gene ID: 114548