Lys20p and Lys21p Antibody (31F5) Summary
| Immunogen |
Lys20p and Lys21p
|
| Specificity |
Lys20p and Lys21p
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Purity |
Tissue culture supernatant
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
This Lys20p and Lys21p (31F5) antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In WB bands can be seen at 47.1 kDa (representing Lys20p) and 48.6 kDa (representing Lys21p).
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
47.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Yeast.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
No buffer
|
| Preservative |
5mM Sodium Azide
|
| Purity |
Tissue culture supernatant
|
Alternate Names for Lys20p and Lys21p Antibody (31F5)
- LYS20 Lys20p
Background
In the yeast Saccharomyces cerevisiae, AAA or alpha-amino adipate pathway is responsible for lysine biosynthesis wherein the first committed step involves condensation of acetyl CoA (AcCoA) with alpha-ketoglutarate (alpha-KG) to generate homocitrate as well as CoA in the nucleus, and this reaction is catalyzed by the homocitrate synthase isozymes encoded by LYS20 and LYS21 which belongs to alpha-IPM synthase/homocitrate synthase family and are 92% identical in amino acid sequence. Expression of both LYS20 and LYS21 is upregulated through Gcn4p upon amino acid deprivation, and their positive regulation is also facilitated by Lys14p (transcriptional activator from Zn-Cys cluster family) with co-inducer alpha-AASA (2-aminoadipate semialdehyde). Moreover, LYS20 and LYS21 are positively co-regulated by target of rapamycin or TOR signalling pathway coupled with protein kinase A, through a LYS14-GCN4-independent pathway. Therefore, LYS20 and LYS21 plays a key role in maintaining levels of lysine, an amino acid preferentially located in the vacuole, therefore not available as a source of nitrogen or carbon source, and lysine has been sown to play important physiological roles: contributing to the build-up of H2O2 triggered stress response, prevention of apoptosis induced upon amino acid starvation, and cryoprotection.