MS4A1/CD20 Antibody (SPM494) – IHC-Prediluted Summary
| Immunogen |
Human tonsil B cells
|
| Localization |
Predominantly cell surface with some cytoplasmic
|
| Marker |
B-Cell Marker
|
| Specificity |
Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
|
| Isotype |
IgG2a Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
MS4A1
|
| Purity |
Tissue culture supernatant
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 11q12.2 Molecular weight of antigen: 33-37kDa Immunohistochemistry-Paraffin 1:100 – 1:200 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
No buffer
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Tissue culture supernatant
|
Alternate Names for MS4A1/CD20 Antibody (SPM494) – IHC-Prediluted
- B1
- B-lymphocyte antigen CD20
- B-lymphocyte cell-surface antigen B1
- B-lymphocyte surface antigen B1
- Bp35
- Bp35MGC3969
- CD20 antigen
- CD20 receptor
- CD20
- CD20S7
- CVID5
- LEU-16
- Leukocyte surface antigen Leu-16
- Ly-44
- Membrane-spanning 4-domains subfamily A member 1
- membrane-spanning 4-domains, subfamily A, member 1
- MS4A1
- MS4A2
- S7