Product: 1alpha, 25-Dihydroxy VD2-D6
Cytokeratin, pan Antibody (KRTL/1077 KRTH/1076) Summary
| Immunogen |
Recombinant human KRT77 and KRT76 protein
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| Localization |
Cytoplasmic
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| Marker |
Epithelial Marker
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| Specificity |
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. KRTL/KRTH is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
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| Isotype |
IgG
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Molecular weight of antigen: 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19)
Immunohistochemistry-Paraffin 0.25 – 0.5 ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. |
Reactivity Notes
Shows broad species reactivity.
Packaging, Storage & Formulations
| Storage |
Store at 4C.
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| Buffer |
10mM PBS and 0.05% BSA
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| Preservative |
0.05% Sodium Azide
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| Purity |
Protein A or G purified
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