Calponin 1 Antibody (CNN1/832)

Product: ARRY-520

Calponin 1 Antibody (CNN1/832) Summary

Immunogen
Recombinant human CNN1 protein
Localization
Cytoplasmic
Marker
Smooth Muscle Marker
Specificity
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CNN1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 – 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 – 1.0 ug/ml
  • Immunofluorescence 1 – 2 ug/ml
Application Notes
Hu-chromosome location: 19p13.2
Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Calponin 1 Antibody (CNN1/832)

  • Basic calponin
  • Calponin 1
  • calponin 1, basic, smooth muscle
  • Calponin H1
  • Calponin H1, smooth muscle
  • calponins, basic
  • CNN1
  • Sm-Calp
  • SMCC
  • SMCCcalponin-1

PMID: 21441599