Goat anti-Rabbit IgG Fc Secondary Antibody [Rhodamine]

Product: Rosiglitazone

Goat anti-Rabbit IgG Fc Secondary Antibody [Rhodamine] Summary

Immunogen
Rabbit IgG F(c) fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.  No reaction was observed against Rabbit IgG F(ab)2 or Human Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 – 1:10000
  • Flow Cytometry 1:1:10 – 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 – 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 – 1:5000

Reactivity Notes

Reacts with Rabbit.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA – Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 570, Excitation Wavelength: 550, Label Molecular Weight: 444, Restore with 1.0 mL of deionized water (or equivalent).

PMID: 2453055