Rabbit anti-Human IgA Secondary Antibody [HRP]

Product: ABT-199

Rabbit anti-Human IgA Secondary Antibody [HRP] Summary

Immunogen
Human IgA a alpha chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgA and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Human heavy or light chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 – 1:5000
  • ELISA 1:56000
  • Immunohistochemistry 1:500 – 1:2500
Readout System
  • NovaLume Plus Chemiluminescent Reagent

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA – Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Human IgA Secondary Antibody [HRP]

  • Arc-1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDHE
  • cell-CAM 120/80
  • ECAD
  • E-cadherin
  • Epithelial cadherin
  • IgA
  • LCAM
  • UVOE-Cadherin
  • uvomorulin

PMID: 25232120