Cbl-c Antibody Summary
| Immunogen |
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 444-458 of Human Cbl-c.
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| Specificity |
A BLAST analysis was used to suggest that this antibody would react with Cbl-c from human and chimpanzee sources. Expect partial reactivity against mouse and rat sources of Cbl-c as approx 83% sequence homology is on record for the immunogen sequence. Reactivity with Cbl-c from other sources has not been determined. No reactivity is expected with Cbl-a or Cbl-b.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
CBLC
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Expect a band at ~52 kDa in size corresponding – Cbl-c by western blotting in the appropriate cell lysate or extract.
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Reactivity Notes
Reactive in human
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Purity |
Immunogen affinity purified
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Alternate Names for Cbl-c Antibody
- Cas-Br-M (murine) ecotropic retroviral transforming sequence c
- Cas-Br-M (murine) ectropic retroviral transforming sequence c
- CBL3
- CBL-3
- CBL-SL
- RING finger protein 57
- RNF57SH3-binding protein CBL-3
- SH3-binding protein CBL-C
- signal transduction protein CBL-C
Background
Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57. Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cbl-c knock out mice show no obvious phenotype. Thus, the physiological role of Cbl-c is not known