Peripherin Antibody Summary
| Immunogen |
Rat Peripherin [UniProt# P21807]
|
| Marker |
Un-myelinated Neurons Marker
|
| Isotype |
IgY
|
| Clonality |
Polyclonal
|
| Host |
Chicken
|
| Gene |
PRPH
|
| Purity |
Ammonium sulfate precipitation
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
This Peripherin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~57 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Rat.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
5mM Sodium Azide
|
| Purity |
Ammonium sulfate precipitation
|
Alternate Names for Peripherin Antibody
- neurofilament 4 (57kD)
- Neurofilament 4
- peripherin
- PRPH1NEF4
Background
Peripherin (PRPH) is a 57kD type III neuronal intermediate filament (NIF) protein which is predominantly expressed in neurons of PNS and in certain CNS neurons specifically from brain stem/spinal cord which generally project toward peripheral structures. Inside developing neurons, PRPH is expressed abundantly in the growth cones and is required for axonal outgrowth as well as maintenance or regeneration. In mature neurons, together with other NIFs, PRPH constitutes a shape-maintaining IF network. Besides its crucial role in development of nervous system, PRPH can also be highly relevant to pathogenesis of neurodegenerative disorder. PRPH expression is induced in CNS neurons (wherein it is not expressed normally) upon brain injury, and factors such as LIF, IL-6 etc and PRPH molecules form aggregates in neurons. Synaptic plasticity is markedly altered in PRPH transgenic mice (Per mice) and up-regulation of PRPH expression is implicated in some pathophysiological conditions, such as cerebral ischemia and seizure, and some cognitive disorders. In ALS patients, PRPH together with neuronal IFs are detected in the majority of abnormal IF inclusion bodies in mature or aging motor neurons.