IL-1 beta/IL-1F2 Antibody [HRP]

Product: Voriconazole

IL-1 beta/IL-1F2 Antibody [HRP] Summary

Description
This antibody was heated to 56 C for 30 minutes.
Immunogen
This antibody was prepared by repeated immunizations with recombinant human IL-1 beta produced in E.coli. The MW of the recombinant 153 aa IL-1 beta was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta converting enzyme (ICE, capase-1).
Specificity
This antibody is primarily directed against mature, 17,000 MW human IL-1 beta.
Clonality
Polyclonal
Host
Rabbit
Gene
IL1B
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody recognizes the 17,000 MW mature IL-1Beta. For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells. PBMC are stimulated for 24 hours with 1% serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG – reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation – stain intracellular IL-1Beta. This antibody is also useful for neutralization of human and primate IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of murine, rat or rabbit IL-1Beta. For neutralization, it is recommended – incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) – Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water to a final concentration of 1.0 mg/ml.

Alternate Names for IL-1 beta/IL-1F2 Antibody [HRP]

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 beta (also known as Interleukin-1 beta, IL-1beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

PMID: 25004203