Recombinant Hepatitis B Surface Antigen adr Protein Summary
| Description |
Glycosylated biologically active polypeptide chains. It migrates with an apparent molecular mass of 23-27 kDa on a reducing SDS-PAGE.
Source: CHO |
| Preparation Method |
Novus biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
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| Details of Functionality |
The biological activity is determined by ELISA with anti-HBsAg antibodies.
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| Protein/Peptide Type |
Biologically Active Protein
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| Purity |
>95% pure by SDS-PAGE
|
| Endotoxin Note |
Less than 1.0 EU/mg of rHBsAgadr as determined by LAL method.
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Applications/Dilutions
| Theoretical MW |
2000 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Purity |
>95% pure by SDS-PAGE
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Notes
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.