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SPRY4 Antibody Summary
| Immunogen |
Synthetic peptide conjugated to KLH found between amino acids 283-340 of Human Sprouty 4.
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| Localization |
Cytoplasmic
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| Clonality |
Polyclonal
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| Host |
Rabbit
|
| Gene |
SPRY4
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
Expect a band ~35 kDa in size corresponding – Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody.
|
Reactivity Notes
A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|
| Preservative |
0.01% Sodium Azide
|
| Purity |
Immunogen affinity purified
|
Alternate Names for SPRY4 Antibody
- protein sprouty homolog 4
- sprouty homolog 4 (Drosophila)
- SPRY4
- spry-4
Background
Sprouty-4 (also known as SPRY4) is an inhibitor of theinsulin receptor and EGFR-transduced mitogen-activated proteinkinase (MAPK) signaling pathway downstream of FGF and EGFreceptor tyrosine kinase activation. It is positioned upstream ofRAS activation and impairs the formation of active GTP-RAS.SPRY4 is widely expressed, with different isoforms. The proteinconsists of 322 amino acids, with a cysteine-rich region, Srchomology 3 binding regions, proline-rich regions, and a PESTsequence. It is expressed predominantly in the cytoplasm.Northern results show bands across most tissues, with strongestexpression in heart, brain, placenta, lung, and intestine.