CD3 epsilon Antibody (APA1/1) [Alexa Fluor® 405]

Product: SCH772984

CD3 epsilon Antibody (APA1/1) [Alexa Fluor® 405] Summary

Immunogen
Purified human CD3 proteins isolated from thymus
Specificity
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD3E
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
This antibody is useful in Flow Cytometry, Immunoprecipitation, Western Blotting, Immunohistochemistry-frozen and Immunocytochemistry

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for CD3 epsilon Antibody (APA1/1) [Alexa Fluor® 405]

  • CD3 epsilon
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

PMID: 6290821