p97/VCP Antibody (Hs-14) [Alexa Fluor® 488]

Product: Forskolin

p97/VCP Antibody (Hs-14) [Alexa Fluor® 488] Summary

Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Specificity
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name “intra-acrosomal protein”.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
VCP
Purity
Size Exclusion Chromatography
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
ICC: Use at an assay dependant dilution. Designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). Membrane permeabilization with acetone is essential. WB: Use at an assay dependant dilution. Detects a band of approximately 220 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Size Exclusion Chromatography

Alternate Names for p97/VCP Antibody (Hs-14) [Alexa Fluor® 488]

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

PMID: 12234612