p97/VCP Antibody (Hs-14) Summary
| Immunogen |
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
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| Specificity |
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name “intra-acrosomal protein”.
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| Isotype |
IgM
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
VCP
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| Purity |
Size Exclusion Chromatography
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Staining technique: Membrane permeabilization (acetone) is essential. Application note: The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
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| Publications |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
TBS (approx. pH 8.0)
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| Preservative |
15mM Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Size Exclusion Chromatography
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Alternate Names for p97/VCP Antibody (Hs-14)
- 15S Mg(2+)-ATPase p97 subunit
- IBMPFD
- IBMPFDMGC8560
- MGC131997
- p97
- TER ATPase
- TERA
- transitional endoplasmic reticulum ATPase
- valosin containing protein
- Valosin-containing proteinMGC148092
- VCP
- yeast Cdc48p homolog
Background
This protein is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The gene encoding this protein consists of 4 exons and its alternative splicing generates 11 distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining 7 transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.