Lson CJ, Emson Pc. Restoration of thalamostriatal projections in rat neostriatal
Lson CJ, Emson Pc. Restoration of thalamostriatal projections in rat neostriatal grafts: electron microscopic evaluation. J Comp Neurol. 1991; 303:224. [PubMed: 2005239] Yung KK, Bolam JP, Smith AD, Hersch SM, Ciliax BJ, Levey AI. Immunocytochemical localization of D1 and D2 dopamine receptors within the basal ganglia with the rat: light and electron microscopy. Neuroscience. 1995; 65:70930. [PubMed: 7609871]NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; readily available in PMC 2014 August 25.Lei et al.ALDH1 MedChemExpress PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 1.Low power (A,B) and 40light microscopic views (C,D) of VGLUT1 (A,C) and VGLUT2 (B,D) immunolabeling of rat brain. Photos A and B present views at a coronal level anterior to the anterior commissure; note the presence of VGLUT2 thalamostriatal terminals in cortical layer 4 in image B and the conspicuous absence of VGLUT1 cortical terminals in cortical layer four in image A. The 40views reveal the presence of numerous varicosities in striatum immunolabeled for VGLUT1 (C) and VGLUT2 (D), using the varicosities much more than twice as abundant for VGLUT1 as VGLUT2.J Comp Neurol. Author manuscript; offered in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure two.CLSM photos in (A,C,E) present a single field from a double-labeled section, HDAC1 custom synthesis showing the comprehensive colocalization of immunolabeling in rat striatum for guinea pig (GP) antiVGLUT2 (A) and rabbit (Rb) anti-VGLUT2 (C), as shown by the full labeling overlap within the merged image (E) for (A,C). Conversely, CLSM photos in (B,D,F) present a single field from a double-labeled section, showing the near absence of colocalization of immunolabeling in rat striatum for guinea pig (GP) anti-VGLUT1 (B) and rabbit (Rb) antiVGLUT2 (D), as shown by the meager overlap inside the merged image (F) for (B,D).J Comp Neurol. Author manuscript; offered in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure three.Detail of every CLSM image shown in Figure 2. Photos in (A,C,E) present magnified views with the upper left from Fig. 2A,C,E, respectively. Similarly, pictures (B,D,F) present magnified views with the upper left from Fig. 2B,D,F, respectively. These magnified views make it extra possible to resolve person terminals, and thereby confirm: 1) the total colocalization noticed in rat striatum for guinea pig (GP) anti-VGLUT2 (A) and rabbit (Rb) anti-VGLUT2 (C), as additional evidenced by the full labeling overlap inside the merged image (E) for (A,C); and 2) the near absence of colocalization in rat striatum for guinea pigJ Comp Neurol. Author manuscript; obtainable in PMC 2014 August 25.Lei et al.Pageanti-VGLUT1 (B) and rabbit anti-VGLUT2 (D), as shown by the absence of evident overlap in the merged image (F) for (B,D).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; accessible in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 4.CLSM views of immunofluorescence for VGLUT1 (A) or VGLUT2 (B) in fields with fluorescent PHAL labeling of corticostriatal axons and terminals (C,D). Note that corticostriatal terminals in (C) immunolabel for VGLUT1 but these corticostriatal terminals in (D) don’t immunolabel for VGLUT2. This could be noticed extra clearly in the mer.