The α9β1 Source angiogenic and therapeutic benefits related with CD34+ stem cell therapy.Trafficking studies applying confocal imaging and flow cytometry analyses revealed that CD34Exo was selectively internalised by endothelial cells and cardiomyocytes relative to fibroblasts in the CD34Exo-injected ischemic hearts. MicroRNA expression profiling and Taqman assays indicated that CD34Exo are substantially enriched with pro-angiogenic miRNAs for example miR126. CD34Exo injection induced the expression of miR126 and several pro-angiogenic mRNAs in mouse ischemic myocardium, suggesting a direct transfer of miR126. CD34Exo lacking in miR126 had decreased angiogenic and therapeutic activity both in vitro and in vivo indicating that miR126 was critical for CD34Exo function.OS20.Mesenchymal stem cells and their secreted exosomes exert therapeutic effects in Duchenne muscular dystrophy Ariel Bier1, Peter Bernstein1, Simona Cazacu2, Amir Dori3 and Chaya Brodie4 Bar-Ilan University, Israel; 2Henry Ford Health Systems, Detroit, MI, USA; Sheba Healthcare Centre, Israel; 4Faculty of Life Sciences Bar-Ilan University, Israel and Neurosurgery Department, Henry Ford Wellness Systems, Detroit, MI, USA3OS20.Angiogenic mechanisms of human CD34+ stem cell exosomes inside the repair of ischemic heart CD40 review Yaxuan Liang1, Prabhu Mathiyalagan1, Sol Misener2, Douglas Losordo3 and Susmita Sahoo1 Cardiovascular Study Center, Icahn College of Medicine at Mount Sinai, New York, USA; 2Feinberg Cardiovascular Research Institute, Feinberg College of Medicine, Northwestern University, NY, USA; 3Caladrius BiosciencesIntroduction: Locally transplanted human CD34+ stem cells happen to be shown to improve physical exercise tolerance in patients with myocardial ischemia and promote angiogenesis in animal models. In an earlier study, very first of its type, we’ve demonstrated that CD34+ cells secrete exosomes (CD34Exo) that constitute a important component in the pro-angiogenic paracrine activity in the cells. Right here, we investigated the mechanisms of CD34Exo-mediated repair of the ischemic myocardium and therapeutic angiogenesis by studying their miRNA content material and uptake.Duchenne muscular dystrophy (DMD) can be a progressive lethal, X-linked illness of skeletal and cardiac muscles caused by mutation on the dystrophin gene, which results in muscle degeneration. Cell therapy employing various cell types has been considered a potential therapeutic approach for the remedy of DMD. Mesenchymal stromal cells (MSCs) are obtained from autologous bone marrow and adipose tissues or from allogeneic placenta and umbilical cord. The safety and therapeutic impact of MSCs happen to be demonstrated in pre-clinical and clinical studies and are attributed to paracrine effects that are partly mediated by extracellular vesicles. Here, we studied the therapeutic effects of MSCs and their secreted exosomes using human in vitro disease models of skeletal muscle cultures derived from healthy and Duchenne individuals and MDX mice. Treatment of satellite cells with conditioned media or exosomes secreted by MSCs improved the proliferation and generation of PAX7+/MyoD+ cells along with the differentiation of human myoblasts from both healthy and DMD individuals. MSCs from various sources exerted differential effects on the function in the muscle cells. Secretome and RNA sequencing evaluation from the MSC-derived exosomes revealed specific cytokines and clusters of miRNAs and extended non-coding RNAs that have been related with anti-inflammatory and pro-regenerative activitie.
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