Bad Antibody (BYC001) Summary
| Immunogen |
Full-length murine Bad containing a N-terminal His6-tag.
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| Specificity |
Recognizes human Bad, Mr 23kD, and mouse Bad, Mr 31kD. Occasionally a Mr 14kD protein is detected in human lysates.
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| Isotype |
IgG
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
BAD
|
| Purity |
Protein G purified
|
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Applications/Dilutions
| Dilutions |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
0.1M Tris-Glycine (pH 7.4) and 0.15M NaCl
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| Preservative |
0.05% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Protein G purified
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Alternate Names for Bad Antibody (BYC001)
- Bad
- BBC2
- BBC6
- bcl2 antagonist of cell death
- BCL2-antagonist of cell death protein
- BCL2-associated agonist of cell death
- Bcl-2-binding component 6
- BCL2-binding component 6
- BCL2-binding protein
- BCL2L8
- Bcl2-L-8
- BCL2L8bcl2-L-8
- Bcl-2-like protein 8
- BCL-X/BCL-2 binding protein
- Bcl-XL/Bcl-2-associated death promoter
Background
Bad is a pro-apoptotic member of the Bcl-2 family. Bad will bind preferentially Bcl-xL over Bcl-2 through its partial (9-16 a.a) BH3 domain. Additionally, Bad can reverse the death repressor activity of Bcl-xL but not of Bcl-2. Upon dephosphorylation, activated Bad translocates to the mitochondria and displace Bcl-2 from tBid sequestration to initiate mitochondrial dysfunction. The switching on/off of its phosphorylation by growth/survival factors regulates Bad activity.